Chimeric chalcone synthase
WebOct 21, 2013 · The I locus is a 27-kb inverted repeat cluster of chalcone synthase genes CHS1-3-4 that mediates siRNA down-regulation of CHS7 and CHS8 target mRNAs during seed development leading to yellow seed coats lacking anthocyanin pigments. Here, we report small RNA sequencing of ten stages of seed development from a few days post … WebAbstract. A chimeric gene was constructed containing the light-inducible chalcone synthase (chs) promoter from Antirrhinum majus, the neomycin phosphotransferase structural sequence from Tn5 as a reporter gene (NPTII) and the termination region from chs gene 1 from Petroselinum hortense.This gene was introduced into tobacco plants with …
Chimeric chalcone synthase
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WebJun 1, 1992 · We developed an Agrobacterium-mediated leaf disk transformation method for chrysanthemum. We introduced a chimeric chalcone synthase (CHS) gene isolated from chrysanthemum into cv. Moneymaker (pink type) to produce white-flowered plants.
WebApr 1, 1993 · Introduction of a chimeric chalcone synthase gene into letunia results in reversible co-suppression of homologous genes in trans. The Plant Cell 2 : 279–289. Article CAS Google Scholar WebApr 1, 1993 · Introduction of a chimeric chalcone synthase gene into letunia results in reversible co-suppression of homologous genes in trans. The Plant Cell 2 : 279–289. …
WebChalcone Synthase Gene. Chalcone synthase (CHS) is the first committed enzyme in flavonoid biosynthesis (Fig. 1) and catalyzes the stepwise condensation of three acetate … WebIn Basic medium (base catalysis), the chalcone is produced from the aldol product via dehydration in (an enolate) - mechanism, while in acidic medium (acid catalysis, it is produced via (an...
Chalcone synthase or naringenin-chalcone synthase (CHS) is an enzyme ubiquitous to higher plants and belongs to a family of polyketide synthase enzymes (PKS) known as type III PKS. Type III PKSs are associated with the production of chalcones, a class of organic compounds found mainly in plants as natural … See more CHS catalysis serves as the initial step for flavonoid biosynthesis. Flavonoids are important plant secondary metabolites that serve various functions in higher plants. These include pigmentation, UV protection, fertility, … See more Subunits CHS exists as a homodimeric protein with each monomer approximately 42-45 kDa in size. Each … See more Metabolic CHS is noncompetitively inhibited by flavanoid pathway products such as naringenin and chalcone naringenin. Despite lack of direct evidence in vivo, flavonoids are believed to accumulate in the cytosol to a … See more CHS belongs to a broader class of enzymes known as type III PKSs. Being the first enzyme of its type to be discovered, all other members are often labeled as “CHS-like.” Most or all of the divergent CHS-like enzymes characterized have arisen from … See more 4-coumaroyl-CoA and three units of malonyl-CoA are converted into three molecules of carbon dioxide, four molecules of coenzyme A and one unit of naringenin chalcone. See more The first step involves a transfer of a coumaroyl moiety from a 4-coumaroyl-CoA starter molecule to Cys164. Next, a series of condensation reactions of three acetate units from malonyl-CoA occurs, each proceeding through an acetyl-CoA carbanion derived … See more CHS, as the first committed step in the flavonoid pathway, facilitate production of flavanoids, isoflavonoid-type phytoalexins and other metabolites to protect the plant from stress. CHS … See more
WebApr 1, 1990 · Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans. Napoli C1, Lemieux C, Jorgensen R Author information Affiliations 1 author 1. DNA Plant Technology Corporation, 6701 San Pablo Avenue, Oakland, California 94608. ORCIDs linked to this article greenridge surgery birmingham e consultWebNational Center for Biotechnology Information greenridge state park camp sitesWebApr 1, 1990 · Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans. C. Napoli, C. Lemieux, R. … fly wezo bootWebMay 3, 2011 · Chalcone synthase (CHS, EC 2.3.1.74) is a key enzyme of the flavonoid/isoflavonoid biosynthesis pathway. Besides being part of the plant developmental program the CHS gene expression is induced in plants under stress conditions such as UV light, bacterial or fungal infection. CHS expression causes accumulation of flavonoid and … flywheel 0.6.5WebA chimeric gene was constructed containing the light‐inducible chalcone synthase (chs) promoter from Antirrhinum majus, the neomycin phosphotransferase structural sequence from Tn5 as a reporter gene (NPTII) and the termination region from chs gene 1 from Petroselinum hortense. green ridge state forest vehicle campingWebThe chalcone synthase is a key enzyme that catalyses the first dedicated reaction of the flavonoid pathway in higher plants. The chs gene and its protein p Chalcone synthase … green ridge state forest trail mapsWebChalcone synthase is a dimeric protein with Mr 78 000–88 000 and probably two identical subunits. The pH optimum of the reaction is in the range 7.5–8.5 for 4-coumaroyl-CoA. … flywhale aircraft