WebThe study aims to assess viability, acrosomal menbrane, integrity and mitochondria membrane potential of sperm separated using a percoll density gradient(45% and 90%) and swim-up methods using Hanwoo epididymal sperm frozen semen. Briefy, motile sperm separated using a percoll gradient and swim-up. 25 μl of sperm dilution from droplets … WebAug 22, 2024 · The excitation wavelengths were λ = 561 nm for TRITC and λ = 491 nm for GFP and fluorescein. The excitation objective was a 10×, 0.3 NA Plan Fluor from Nikon, while the detection objective was ...
Fluorescent dextran derivatives – Applications TdB Labs
WebTetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for … WebMar 4, 2024 · We offer individual filters and filter sets targeted at common fluorophores: BFP, CFP, WGFP, GFP, FITC, Alexa Fluor ® 488, YFP, tdTomato, TRITC, Texas Red, mCherry, and Cyanine (CY3.5). In … easy eats recipes on tv
Tetramethylrhodamine - an overview ScienceDirect Topics
Web17th Dec, 2014. Lorenzo P Menzel. if you used more than 1% (para)formalhyde or glutaraldehyde to fix your cells you will see green fluorescence due to the aldehydes. this tidbit is in addition to ... WebFluorescein 494 518 Fluorescein Iso-thiocyanate (FITC) 490 525 Fluo 3 485 503 FM1-43 479 ... Lucifer Yellow VS 430 535 LysoSensor Blue DND-192, DND-167 374 425 LysoSensor Green DND-153, DND-189 ... TRITC (Tetramethyl Rhodamine Isothiocyanate) 557 576 Texas Red 596 615 Thiazine Red R ... WebOur dual fluorescent dextran derivatives are produced by conjugating two fluorophores, namely FITC and TRITC as well as FITC and AR onto a dextran molecule through thiourethane linkages. The degree of substitution (DS) lies in the range of 0.001 to 0.020 mol of dye per mol of glucose units for each of the fluorophores. Read more easyeats xyz